|
KMID : 1001020100080030122
|
|
Korean Journal of Urological Oncology
2010 Volume.8 No. 3 p.122 ~ p.127
|
|
|
Migration Ability of Cytosine Deaminase Gene Expressing Human Neural Stem Cells Toward Prostate Cancer
|
|
Song Yun-Seob
Lee Hong-Jun
Doo Seung-Whan
Yang Won-Jae
Kim Dae-Hong
Lee Sang-Jin
Kim Seung-Up
|
|
|
Abstract
|
|
|
Purpose: Stem cells have migration ability toward cancer cells and therapeutic genes can be easily transduced into stem cells with viral vectors. Cystosine deaminase (CD) is the enzyme that transforms the prodrug 5- fluorocytosine to highly toxic drug 5-fluorouracil (5-FU). This study was performed to investigate the migration ability of CD gene transduced human neural stem cells (Hb1.F3-CD) toward prostate cancer after systemic treatment in C57B mice.
Materials and Methods : An Hb1.F3 with a retroviral vector encoding v-myc, lacZ was generated and transfected with recombinant retrovirus from the pMSCV-puro/CD. Reverse transcription (RT) was performed to evaluate the expression of CD in Hb1.F3-CD. In vivo migration assay after systemic injection of Hb1.F3-CD was performed in prostate cancer induced C57B mice using molecular magnetic resonance (MR) imaging and immunochemical staining.
Results: Transgene expression in CD-hNSCs was demonstrated. In vivo migration ability of CD-hNSCs cells toward TRAMPC2 induced prostate cancer was found using MR imaging and X-gal stain after implantation of HB1.F3-CD in C57B mice.
Conclusions : We confirmed the migration ability of HB1.F3-CD toward prostate cancer cells after systemic treatment in C57B mice. This finding may imply a possible novel selective chemotherapeutic strategy against prostate cancer.
|
|
|
KEYWORD
|
|
|
Stem cell, Prostate cancer, Migration, Gene, Cytosine deaminase
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|